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验证数据展示

  • WB analysis of L02 using 11932-1-AP

    WB analysis of L02 using 11932-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 11932-1-AP

    IHC staining of human liver using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 11932-1-AP

    IHC staining of human liver using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO验证

PRAP1 Polyclonal antibody

PRAP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11932-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11932-1-AP targets PRAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 PRAP1 fusion protein Ag2526 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline-rich acidic protein 1
别名 PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA
计算分子量 151 aa, 17 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC029447
基因名称 PRAP1
Gene ID (NCBI) 118471
RRIDAB_10951856
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NZ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

实验方案

Product Specific Protocols
WB protocol for PRAP1 antibody 11932-1-APDownload protocol
IHC protocol for PRAP1 antibody 11932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Adv Sci (Weinh)

Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

Authors - Qiumo Zhang
  • KD Validated
View Article
human,mouseWB,IF

Nat Commun

Single-cell RNA sequencing reveals placental response under environmental stress

Authors - Eric Van Buren
View Article