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  • Featured Product
  • KD/KO Validated

PRAP1 Polyclonal antibody

PRAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11932-1-AP

Print datasheet

Synonyms

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

验证数据展示

  • WB analysis of L02 using 11932-1-AP

    WB analysis of L02 using 11932-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 11932-1-AP

    IHC staining of human liver using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 11932-1-AP

    IHC staining of human liver using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inL02 cells, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11932-1-AP targets PRAP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PRAP1 fusion protein Ag2526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proline-rich acidic protein 1
Synonyms PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC029447
Gene Symbol PRAP1
Gene ID (NCBI) 118471
RRIDAB_10951856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

实验方案

Product Specific Protocols
WB protocol for PRAP1 antibody 11932-1-APDownload protocol
IHC protocol for PRAP1 antibody 11932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

Adv Sci (Weinh)

Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

Authors - Qiumo Zhang
  • KD Validated
View Article
human,mouseWB,IF

Nat Commun

Single-cell RNA sequencing reveals placental response under environmental stress

Authors - Eric Van Buren
View Article