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验证数据展示

  • WB analysis of MCF-7 using 21097-1-AP

    WB analysis of MCF-7 using 21097-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21097-1-AP

    IHC staining of human liver using 21097-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21097-1-AP

    IHC staining of human liver using 21097-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 21097-1-AP

    IF Staining of HeLa using 21097-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21097-1-AP (PRAS40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

PRAS40 Polyclonal antibody

PRAS40 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21097-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

AKT1S1, AKTS1, Lobe, PRAS40, Proline rich AKT1 substrate 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21097-1-AP targets PRAS40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 PRAS40 fusion protein Ag15355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AKT1 substrate 1 (proline-rich)
别名 AKT1S1, AKTS1, Lobe, PRAS40, Proline rich AKT1 substrate 1
计算分子量 27 kDa
观测分子量 40 kDa
GenBank蛋白编号BC051844
基因名称 PRAS40
Gene ID (NCBI) 84335
RRIDAB_2878811
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRAS40 antibody 21097-1-APDownload protocol
IHC protocol for PRAS40 antibody 21097-1-APDownload protocol
IF protocol for PRAS40 antibody 21097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

Hypothalamic SLC7A14 accounts for aging-reduced lipolysis in white adipose tissue of male mice

Authors - Xiaoxue Jiang
View Article
mouseWB

J Neuroinflammation

Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain

Authors - Yun Li
View Article
humanWB

Front Oncol

The Molecular Landscape and Biological Alterations Induced by PRAS40-Knockout in Head and Neck Squamous Cell Carcinoma.

Authors - Gang Chen
  • KO Validated
View Article