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  • Featured Product
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PRC1 Polyclonal antibody

PRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10110-2-AP

Print datasheet

Synonyms

ASE1, PRC1

验证数据展示

  • WB analysis of MCF-7 using 10110-2-AP

    WB analysis of MCF-7 using 10110-2-AP

    WB result of PRC1 antibody (10110-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

  • WB analysis using 10110-2-AP

    WB analysis using 10110-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10110-2-AP (PRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 10110-2-AP

    IHC staining of human colon cancer using 10110-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10110-2-AP (PRC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 10110-2-AP

    IHC staining of human colon cancer using 10110-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10110-2-AP (PRC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10110-2-AP targets PRC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen PRC1 fusion protein Ag0163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein regulator of cytokinesis 1
Synonyms ASE1, PRC1
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC005140
Gene Symbol PRC1
Gene ID (NCBI) 9055
RRIDAB_2252888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43663
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRC1 is involved in cytokinesis. PRC1 is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a good substrate for several cyclin-dependent kinases (CDKs) in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, suggesting that PRC1 is an in vivo CDK substrate.

实验方案

Product Specific Protocols
WB protocol for PRC1 antibody 10110-2-APDownload protocol
IHC protocol for PRC1 antibody 10110-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immun Inflamm Dis

E2F3 renders an immunosuppressive tumor microenvironment in nasopharyngeal carcinoma: Involvements of the transcription activation of PRC1 and BIRC5

Authors - Qiang Wang
View Article