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验证数据展示

  • WB analysis of HEK-293 using 23827-1-AP

    WB analysis of HEK-293 using 23827-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23827-1-AP (PRDM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 23827-1-AP

    IP experiment of mouse testis using 23827-1-AP

    IP result of anti-PRDM10 (IP:23827-1-AP, 5ug; Detection:23827-1-AP 1:500) with mouse testis tissue lysate 8000ug.

PRDM10 Polyclonal antibody

PRDM10 Polyclonal Antibody for WB, IP, ELISA
Cat No. 23827-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

KIAA1231, PFM7, PR domain containing 10, PRDM10, TRIS, Tristanin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23827-1-AP targets PRDM10 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 PRDM10 fusion protein Ag20605 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PR domain containing 10
别名 KIAA1231, PFM7, PR domain containing 10, PRDM10, TRIS, Tristanin
计算分子量 1156 aa, 131 kDa
观测分子量 120-150 kDa
GenBank蛋白编号BC112934
基因名称 PRDM10
Gene ID (NCBI) 56980
RRIDAB_2879330
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQV6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDM10 is a member of PRDM family, which contains a PR(PRDI-BF1 and RIZ homology) domain, and PRDM is also a subgroup of PR/SET transcription factor family for its PR domain is similar to the catalytic motif of the SET domain of histone methyltransferase(HMTase). Thus PRDM is suggested involve in the regulation of genome expression and chromatin remodeling. PRDM10 shares characteristics with the PR/SET faimly and clusters of zinc fingers DNA binding motifs. It's postualated PRDM10 has an essential role in regulating gene expression and tissue differentiation and a gene repressor. It may play a role in the pathogenesis of gangliosidosis.

实验方案

Product Specific Protocols
WB protocol for PRDM10 antibody 23827-1-APDownload protocol
IP protocol for PRDM10 antibody 23827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols