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PRDM13 Polyclonal antibody

PRDM13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30453-1-AP

Print datasheet

Synonyms

PR domain containing 13, PFM10, MU MB 20.220

验证数据展示

  • WB analysis using 30453-1-AP

    WB analysis using 30453-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30453-1-AP (PRDM13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat eye using 30453-1-AP

    IHC staining of rat eye using 30453-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 30453-1-AP (PRDM13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat eye using 30453-1-AP

    IHC staining of rat eye using 30453-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 30453-1-AP (PRDM13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30453-1-AP targets PRDM13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRDM13 fusion protein Ag31014 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PR domain containing 13
Synonyms PR domain containing 13, PFM10, MU MB 20.220
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberNM_021620
Gene Symbol PRDM13
Gene ID (NCBI) 59336
RRIDAB_3086321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4Q3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM13 antibody 30453-1-APDownload protocol
IHC protocol for PRDM13 antibody 30453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols