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验证数据展示

  • WB analysis using 30453-1-AP

    WB analysis using 30453-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30453-1-AP (PRDM13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat eye using 30453-1-AP

    IHC staining of rat eye using 30453-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 30453-1-AP (PRDM13 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat eye using 30453-1-AP

    IHC staining of rat eye using 30453-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 30453-1-AP (PRDM13 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRDM13 Polyclonal antibody

PRDM13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30453-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PR domain containing 13, PFM10, MU MB 20.220

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30453-1-AP targets PRDM13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRDM13 fusion protein Ag31014 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PR domain containing 13
别名 PR domain containing 13, PFM10, MU MB 20.220
计算分子量 74 kDa
观测分子量 85 kDa
GenBank蛋白编号NM_021620
基因名称 PRDM13
Gene ID (NCBI) 59336
RRIDAB_3086321
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4Q3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM13 antibody 30453-1-APDownload protocol
IHC protocol for PRDM13 antibody 30453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols