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PRDM16 Polyclonal antibody

PRDM16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55361-1-AP

Print datasheet

Synonyms

MEL1, MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16

验证数据展示

  • WB analysis using 55361-1-AP

    WB analysis using 55361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55361-1-AP (PRDM16 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 55361-1-AP

    IHC staining of human stomach using 55361-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55361-1-AP (PRDM16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRDM16 antibody included

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, NCI-H1299 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

PRDM16 has low expression in most cells and tissues

Planning an IHC experiment? We recommend our IHCeasy PRDM16 Ready-To-Use IHC Kit. PRDM16 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55361-1-AP targets PRDM16 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PR domain containing 16
Synonyms MEL1, MDS1/EVI1-like gene 1, MDS1/EVI1 like gene 1, KIAA1675, Histone-lysine N-methyltransferase PRDM16
Calculated Molecular Weight 140 kDa
Observed Molecular Weight~150 kDa
GenBank Accession NumberNM_022114
Gene Symbol PRDM16
Gene ID (NCBI) 63976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM16 antibody 55361-1-APDownload protocol
IHC protocol for PRDM16 antibody 55361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols