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验证数据展示

  • WB analysis of HeLa using 27718-1-AP

    WB analysis of HeLa using 27718-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27718-1-AP (PRDM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PRDM2 Polyclonal antibody

PRDM2 Polyclonal Antibody for WB, ELISA
Cat No. 27718-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

GATA 3 binding protein G3B, HUMHOXY1, KMT8, Lysine N methyltransferase 8, MTB ZF, MTE binding protein, PR domain containing protein 2, PRDM2, RIZ, RIZ1, RIZ2, Zinc finger protein RIZ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27718-1-AP targets PRDM2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 PRDM2 fusion protein Ag26769 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PR domain containing 2, with ZNF domain
别名 GATA 3 binding protein G3B, HUMHOXY1, KMT8, Lysine N methyltransferase 8, MTB ZF, MTE binding protein, PR domain containing protein 2, PRDM2, RIZ, RIZ1, RIZ2, Zinc finger protein RIZ
计算分子量 189 kDa
观测分子量 250-280 kDa
GenBank蛋白编号NM_001007257
基因名称 PRDM2
Gene ID (NCBI) 7799
RRIDAB_2880951
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRDM2, also named as KMT8, RIZ, is a 1718 amino acid protein, which belongs to the class V-like SAM-binding methyltransferase superfamily. PRDM2 localizes in the nucleus and is highly expressed in retinoblastoma cell lines and in brain tumors and is also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3. PRDM2 as a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. The calculated molecular weight of PRDM2 is about 189 kDa. PRDM2 exists some isoforms with 250 kDa and 280 kDa. (PMID: 26040698)

实验方案

Product Specific Protocols
WB protocol for PRDM2 antibody 27718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols