验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, COLO 320 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13792-1-AP targets PRDM4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PRDM4 fusion protein Ag4580 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PR domain containing 4 |
| 别名 | PFM1, PR domain containing 4, PR domain containing protein 4, PRDM4 |
| 计算分子量 | 801 aa, 88 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC035581 |
| 基因名称 | PRDM4 |
| Gene ID (NCBI) | 11108 |
| RRID | AB_10638307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKN5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PRDM family members are characterized by the presence of a PR (positive regulatory) domain and multiple zinc finger (ZnFg) domains. The PR domain defines a family of transcription factors involved in cell differentiation and tumorigenesis. PRDM proteins are either epigenetic modifiers in their own right or else they recruit third party chromatin modifiers - e.g. histone deacetylases (HDACs), histone lysine MTases or histone arginine MTases - to regulate cell type-specific gene expression in various tissues (PRDM4 as an HDAC-associated transcriptional repressor that modulates cell cycle progression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRDM4 antibody 13792-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
