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PRDM7 Polyclonal antibody

PRDM7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22078-1-AP

Print datasheet

Synonyms

PFM4, PR domain containing 7, PR domain containing protein 7, PRDM7, PRDM9

验证数据展示

  • WB analysis of HeLa using 22078-1-AP

    WB analysis of HeLa using 22078-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22078-1-AP (PRDM7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 22078-1-AP

    IHC staining of mouse testis using 22078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22078-1-AP (PRDM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22078-1-AP targets PRDM7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PRDM7 fusion protein Ag17226 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PR domain containing 7
Synonyms PFM4, PR domain containing 7, PR domain containing protein 7, PRDM7, PRDM9
Calculated Molecular Weight 171 aa, 18, 46 kDa
Observed Molecular Weight 46 kDa, 66 kDa
GenBank Accession NumberBC107033
Gene Symbol PRDM7
Gene ID (NCBI) 11105
RRIDAB_2878986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM7 antibody 22078-1-APDownload protocol
IHC protocol for PRDM7 antibody 22078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols