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验证数据展示

  • WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

    WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66820-1-Ig (same clone as 66820-1-PBS)

    WB analysis of HEK-293 using 66820-1-Ig (same clone as 66820-1-PBS)

    WB result of PRDX1 antibody (66820-1-Ig; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 66820-1-Ig (same clone as 66820-1-PBS)

    WB analysis of U2OS using 66820-1-Ig (same clone as 66820-1-PBS)

    U2OS (WT and PRDX1 KO) lysates prepared with RIPA buffer, 10 μg protein loaded. 66820-1-Ig incubated at 1:5000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    IF Staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using PRDX1 antibody (66820-1-Ig, Clone: 2B2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

    WB analysis using 66820-1-Ig (same clone as 66820-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66820-1-Ig (PRDX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    IHC staining of human liver cancer using 66820-1-Ig (same clone as 66820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66820-1-Ig (PRDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66820-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PRDX1 Monoclonal antibody, PBS Only

PRDX1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66820-1-PBS

产品说明书

CloneNo. 2B2A2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

PAG, NKEF-A, Natural killer cell-enhancing factor A, MSP23, EC:1.11.1.24

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66820-1-PBS targets PRDX1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRDX1 fusion protein Ag8821 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 peroxiredoxin 1
别名 PAG, NKEF-A, Natural killer cell-enhancing factor A, MSP23, EC:1.11.1.24
计算分子量 199 aa, 22 kDa
观测分子量 23 kDa
GenBank蛋白编号BC007063
基因名称 PRDX1
Gene ID (NCBI) 5052
RRIDAB_2882163
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ06830
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 ((PMID: 9497357)). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).