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  • Featured Product
  • KD/KO Validated

PRDX3 Recombinant antibody, PBS Only

PRDX3 Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L11

Cat No : 81833-1-PBS

Print datasheet

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

验证数据展示

  • WB analysis using 81833-1-RR (same clone as 81833-1-PBS)

    WB analysis using 81833-1-RR (same clone as 81833-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81833-1-RR (PRX3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

    IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX3 antibody included
  • IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

    IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX3 antibody included
  • WB analysis of HEK-293 using 81833-1-RR (same clone as 81833-1-PBS)

    WB analysis of HEK-293 using 81833-1-RR (same clone as 81833-1-PBS)

    WB result of PRDX3 antibody (81833-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Affinity experiments of 81833-1-RR

    Affinity experiments of 81833-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81833-1-RR against Human PRDX3 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy PRDX3 Ready-To-Use IHC Kit. PRDX3 primary antibody included.

产品信息

81833-1-PBS targets PRDX3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PRDX3 fusion protein Ag1062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name peroxiredoxin 3
Synonyms Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22
Calculated Molecular Weight 27 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30048
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.