验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, HepG2 cells, U-937 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17724-1-AP targets PRX5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PRX5 fusion protein Ag12118 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | peroxiredoxin 5 |
| 别名 | ACR1, Alu corepressor 1, Antioxidant enzyme B166, AOEB166, AOPP, B166, Liver tissue 2D page spot 71B, Peroxiredoxin 5, Peroxiredoxin 5, mitochondrial, Peroxiredoxin V, Peroxisomal antioxidant enzyme, PLP, PMP20, PRDX5, Prx V, PRX5, PRXV, SBBI10, Thioredoxin peroxidase PMP20, Thioredoxin reductase, TPx type VI |
| 计算分子量 | 214 aa, 22 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC110983 |
| 基因名称 | PRX5 |
| Gene ID (NCBI) | 25824 |
| RRID | AB_2168628 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30044 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRX5 antibody 17724-1-AP | Download protocol |
| IHC protocol for PRX5 antibody 17724-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice | ||
Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. | ||
FASEB J Antioxidant enzyme peroxiredoxin 5 regulates cyst growth and ciliogenesis via modulating Plk1 stability.
| ||
Bioorg Chem Novel [1,2,3]triazolo[4,5-d]pyrimidine derivatives containing hydrazone fragment as potent and selective anticancer agents. | ||
J Biol Chem mTOR Inhibition with Rapamycin Improves Cardiac Function in Type 2 Diabetic Mice: Potential Role of Attenuated Oxidative Stress and Altered Contractile Protein Expression. |
