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0
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验证数据展示

  • WB analysis using 13585-1-AP (same clone as 13585-1-PBS)

    WB analysis using 13585-1-AP (same clone as 13585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    WB analysis of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    HAP1 (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 13585-1-AP incubated at 1:2000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    WB analysis of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    WB result of PRDX6 antibody (13585-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • WB analysis using 13585-1-AP (same clone as 13585-1-PBS)

    WB analysis using 13585-1-AP (same clone as 13585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • IP experiment of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    IP experiment of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 13585-1-AP coupled to protein A- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    IP experiment of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    IP result of anti-PRDX6 (IP:13585-1-AP, 3ug; Detection:13585-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • IF Staining of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    IF Staining of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 13585-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX6 antibody included
  • IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX6 antibody included
  • IHC staining of human colon using 13585-1-AP (same clone as 13585-1-PBS)

    IHC staining of human colon using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX6 antibody included
  • IHC staining of human colon using 13585-1-AP (same clone as 13585-1-PBS)

    IHC staining of human colon using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRDX6 antibody included
  • IF Staining of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    IF Staining of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13585-1-AP (same clone as 13585-1-PBS)

    IF Staining of HepG2 using 13585-1-AP (same clone as 13585-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PRDX6 Polyclonal antibody, PBS Only

PRDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13585-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EC:2.3.1.23, EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 1-Cys PRX

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRDX6 Ready-To-Use IHC Kit. PRDX6 primary antibody included.

产品信息

13585-1-PBS targets PRDX6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRDX6 fusion protein Ag4512 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peroxiredoxin 6
别名 EC:2.3.1.23, EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 1-Cys PRX
计算分子量 224 aa, 25 kDa
观测分子量 25-30 kDa
GenBank蛋白编号BC035857
基因名称 PRDX6
Gene ID (NCBI) 9588
RRIDAB_2168637
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30041
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).