验证数据展示
经过测试的应用
| Positive WB detected in | human lung tissue, Jurkat cells, mouse lung tissue |
| Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11536-1-AP targets PREP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, hamster |
| 免疫原 | PREP fusion protein Ag2101 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prolyl endopeptidase |
| 别名 | Post-proline cleaving enzyme, PEP, EC:3.4.21.26 |
| 计算分子量 | 710 aa, 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | BC030636 |
| 基因名称 | PREP |
| Gene ID (NCBI) | 5550 |
| RRID | AB_2284249 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48147 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PREP antibody 11536-1-AP | Download protocol |
| IHC protocol for PREP antibody 11536-1-AP | Download protocol |
| IF protocol for PREP antibody 11536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gut Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease. | ||
Oxid Med Cell Longev Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration.
| ||
Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. | ||
PLoS One Cigarette smoke-induced collagen destruction; key to chronic neutrophilic airway inflammation? | ||
Am J Physiol Lung Cell Mol Physiol Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown. | ||
J Gen Virol Phosphorylation of the Autographa californica multiple nucleopolyhedrovirus polyhedron envelope protein plays an important role in the formation of the occlusion body envelope. |
