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PREPL Polyclonal antibody

PREPL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12478-1-AP

Print datasheet

Synonyms

KIAA0436, PPCEL, PREPL, prolyl endopeptidase like, Prolylendopeptidase like

验证数据展示

  • WB analysis of human brain using 12478-1-AP

    WB analysis of human brain using 12478-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 12478-1-AP

    WB analysis of mouse brain using 12478-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12478-1-AP (PREPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12478-1-AP

    IHC staining of mouse brain using 12478-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 12478-1-AP

    IHC staining of rat brain using 12478-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12478-1-AP (PREPL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12478-1-AP targets PREPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PREPL fusion protein Ag3196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prolyl endopeptidase-like
Synonyms KIAA0436, PPCEL, PREPL, prolyl endopeptidase like, Prolylendopeptidase like
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC013193
Gene Symbol PREPL
Gene ID (NCBI) 9581
RRIDAB_2300040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4J6C6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PREPL (Prolyl endopeptidase-like) is also named as KIAA0436 and belongs to the prolyl oligopeptidase subfamily of serine peptidases. IPREPL is a novel oligopeptidase with unique structural and functional characteristics (PMID:16385448). It has 4 isoforms produced by alternative splicing and can exsit as a homodimer (PMID:16143824). Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS).

实验方案

Product Specific Protocols
WB protocol for PREPL antibody 12478-1-APDownload protocol
IHC protocol for PREPL antibody 12478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols