Perforin Polyclonal antibody

Perforin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 14580-1-AP

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Synonyms

Perforin 1, P1, Lymphocyte pore-forming protein, HPLH2, FLH2



经过测试的应用

Positive WB detected inCTLL-2 cells, mouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14580-1-AP targets Perforin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, chicken
Immunogen Perforin fusion protein Ag6060 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name perforin 1 (pore forming protein)
Synonyms Perforin 1, P1, Lymphocyte pore-forming protein, HPLH2, FLH2
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC063043
Gene Symbol Perforin
Gene ID (NCBI) 5551
RRIDAB_10639524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Perforin 1 (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)

实验方案

Product Specific Protocols
WB protocol for Perforin antibody 14580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF, WB

Front Cell Infect Microbiol

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Teng Li
humanWB

Mol Immunol

VEGF165b augments NK92 cytolytic activity against human K562 leukemia cells by upregulating the levels of perforin and granzyme B via the VEGR1-PLC pathway.

Authors - Xiaoyin Wang
chickenWB

Res Vet Sci

Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius.

Authors - Cui-Ying Chen
mouseIF

J Exp Clin Cancer Res

Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells.

Authors - Chengguo Li
mouseIF

Biomed Pharmacother

Tannins in Terminalia bellirica inhibits hepatocellular carcinoma growth via re-educating tumor-associated macrophages and restoring CD8+T cell function

Authors - Zihao Chang
humanWB

J Immunol Res

The SIX1/LDHA Axis Promotes Lactate Accumulation and Leads to NK Cell Dysfunction in Pancreatic Cancer

Authors - Wanli Ge