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PRH1 Polyclonal antibody

PRH1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21460-1-AP

Print datasheet

Synonyms

PRPC, Peptide P-C, Parotid proline-rich protein 1/2, Parotid double-band protein, Pa

验证数据展示

  • WB analysis of HepG2 using 21460-1-AP

    WB analysis of HepG2 using 21460-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21460-1-AP (PRH1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of HepG2 using 21460-1-AP

    IF Staining of HepG2 using 21460-1-AP

    Immunofluorescent analysis of HepG2 cells using 21460-1-AP (PRH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21460-1-AP targets PRH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PRH1 fusion protein Ag14833 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name proline-rich protein HaeIII subfamily 1
Synonyms PRPC, Peptide P-C, Parotid proline-rich protein 1/2, Parotid double-band protein, Pa
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC064553
Gene Symbol PRH1
Gene ID (NCBI) 5554
RRIDAB_2878862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRH1, also known as Salivary acidic proline-rich phosphoprotein 1/2, is a 166 amino acid secreted protein whose molecular weight is 17 kDa. It functions as a highly potent inhibitor of calcium phosphate crystal growth. Similar to other PRPs, PRH1 provides a protective and reparative environment for dental enamel, which is important for teeth integrity. This antibody specifically recognises the 17 kDa protein.

实验方案

Product Specific Protocols
WB protocol for PRH1 antibody 21460-1-APDownload protocol
IF protocol for PRH1 antibody 21460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols