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PRIM2 Polyclonal antibody

PRIM2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11788-1-AP

Print datasheet

Synonyms

PRIM2A, p58

验证数据展示

  • WB analysis using 11788-1-AP

    WB analysis using 11788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11788-1-AP (PRIM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 11788-1-AP

    IF Staining of A375 using 11788-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 11788-1-AP (PRIM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11788-1-AP targets PRIM2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRIM2 fusion protein Ag2370 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name primase, DNA, polypeptide 2 (58kDa)
Synonyms PRIM2A, p58
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC017833
Gene Symbol PRIM2
Gene ID (NCBI) 5558
RRIDAB_2169380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRIM2 antibody 11788-1-APDownload protocol
IF protocol for PRIM2 antibody 11788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols