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验证数据展示

  • WB analysis of HeLa using 67491-1-Ig (same clone as 67491-1-PBS)

    WB analysis of HeLa using 67491-1-Ig (same clone as 67491-1-PBS)

    WB result of PRKACA antibody (67491-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:2300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKACA antibody included
  • IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKACA antibody included
  • Featured Product
  • KD/KO验证

PRKACA Monoclonal antibody, PBS Only

PRKACA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67491-1-PBS

产品说明书

CloneNo. 4G10D7

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, ELISA

别名

PKA C alpha, PKACA, PRKACA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKACA Ready-To-Use IHC Kit. PRKACA primary antibody included.

产品信息

67491-1-PBS targets PRKACA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 PRKACA fusion protein Ag26400 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, catalytic, alpha
别名 PKA C alpha, PKACA, PRKACA
计算分子量 41 kDa
观测分子量 38-43 kDa
GenBank蛋白编号BC039846
基因名称 PRKACA
Gene ID (NCBI) 5566
RRIDAB_2882716
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17612
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.