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PRKACB Polyclonal antibody, PBS Only

PRKACB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12232-1-PBS

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Synonyms

验证数据展示

  • WB analysis of MCF-7 using 12232-1-AP (same clone as 12232-1-PBS)

    WB analysis of MCF-7 using 12232-1-AP (same clone as 12232-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKACB antibody included
  • IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    IHC staining of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKACB antibody included
  • IP experiment of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    IP experiment of mouse brain using 12232-1-AP (same clone as 12232-1-PBS)

    IP result of anti-PRKACB (IP:12232-1-AP, 4ug; Detection:12232-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12232-1-AP (same clone as 12232-1-PBS)

    IF Staining of HeLa using 12232-1-AP (same clone as 12232-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKACB antibody (12232-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12232-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRKACB Ready-To-Use IHC Kit. PRKACB primary antibody included.

产品信息

12232-1-PBS targets PRKACB in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRKACB fusion protein Ag2870 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, catalytic, beta
Synonyms
Calculated Molecular Weight 41 kDa, 46 kDa
Observed Molecular Weight 36-55 kDa
GenBank Accession NumberBC016285
Gene Symbol PRKACB
Gene ID (NCBI) 5567
RRIDAB_2170177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22694
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.