• Featured Product
  • KD/KO验证

PRKAR2A Polyclonal antibody

PRKAR2A Polyclonal Antibody for WB, IF-P, IP, ELISA
Cat No. 10142-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, ELISA and More (1)

PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue, PC-3 cells, human testis tissue
Positive IP detected inHeLa cells, HeLa cells
Positive IF-P detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy PRKAR2A Ready-To-Use IHC Kit. PRKAR2A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10142-2-AP targets PRKAR2A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 PRKAR2A fusion protein Ag0203 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type II, alpha
别名 PRKAR2, PKR2, cAMP-dependent protein kinase type II-alpha regulatory subunit
计算分子量 43 kDa
观测分子量 46-52 kDa
GenBank蛋白编号BC002763
基因名称 PRKAR2A
Gene ID (NCBI) 5576
RRIDAB_2168236
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13861
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

实验方案

Product Specific Protocols
WB protocol for PRKAR2A antibody 10142-2-APDownload protocol
IF protocol for PRKAR2A antibody 10142-2-APDownload protocol
IP protocol for PRKAR2A antibody 10142-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

Depletion of LAMP3 enhances PKA-mediated VASP phosphorylation to suppress invasion and metastasis in esophageal squamous cell carcinoma.

Authors - Furong Huang
  • KD Validated
ratWB,IHC

Life Sci

TMT-based proteomics analysis to screen potential biomarkers of acute-phase TBI in rats.

Authors - Haochen Wang
human,mouseWB,IP

Cell Rep

STK25 inhibits PKA signaling by phosphorylating PRKAR1A

Authors - Xiaokan Zhang
mouseWB

J Transl Med

Nicotine restores olfactory function by activation of prok2R/Akt/FoxO3a axis in Parkinson's disease

Authors - Qinglong Guo
Loading...