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验证数据展示

  • WB analysis using 20845-1-AP

    WB analysis using 20845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20845-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 20845-1-AP

    IHC staining of human stomach cancer using 20845-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20845-1-AP (PRKAR2B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20845-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, ELISA

别名

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20845-1-AP targets PRKAR2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原 PRKAR2B fusion protein Ag11866 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type II, beta
别名 RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit
计算分子量 418 aa, 46 kDa
GenBank蛋白编号BC075800
基因名称 PRKAR2B
Gene ID (NCBI) 5577
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31323
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKAR2B antibody 20845-1-APDownload protocol
IHC protocol for PRKAR2B antibody 20845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols