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PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20845-1-AP

Print datasheet

Synonyms

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

验证数据展示

  • WB analysis using 20845-1-AP

    WB analysis using 20845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20845-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 20845-1-AP

    IHC staining of human stomach cancer using 20845-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20845-1-AP (PRKAR2B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20845-1-AP targets PRKAR2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, zebrafish
Immunogen PRKAR2B fusion protein Ag11866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, regulatory, type II, beta
Synonyms RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit
Calculated Molecular Weight 418 aa, 46 kDa
GenBank Accession NumberBC075800
Gene Symbol PRKAR2B
Gene ID (NCBI) 5577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31323
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKAR2B antibody 20845-1-APDownload protocol
IHC protocol for PRKAR2B antibody 20845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols