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  • Featured Product
  • KD/KO Validated

PRKCSH Polyclonal antibody, PBS Only

PRKCSH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12148-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IP result of anti-PRKCSH (IP:12148-1-AP, 4ug; Detection:12148-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCSH antibody included
  • IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCSH antibody included
  • IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12148-1-AP (PRKCSH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

    IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCSH antibody included
Planning an IHC experiment? We recommend our IHCeasy PRKCSH Ready-To-Use IHC Kit. PRKCSH primary antibody included.

产品信息

12148-1-PBS targets PRKCSH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRKCSH fusion protein Ag2796 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C substrate 80K-H
Synonyms
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC013586
Gene Symbol PRKCSH
Gene ID (NCBI) 5589
RRIDAB_2253107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14314
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.