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PRKD1 Polyclonal antibody

PRKD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20714-1-AP

Print datasheet

Synonyms

Serine/threonine protein kinase D1, PRKCM, PKD1, PKD

验证数据展示

  • WB analysis of LNCaP using 20714-1-AP

    WB analysis of LNCaP using 20714-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 20714-1-AP (PRKD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 20714-1-AP

    IHC staining of rat cerebellum using 20714-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 20714-1-AP (PRKD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKD1 antibody included
  • IHC staining of rat cerebellum using 20714-1-AP

    IHC staining of rat cerebellum using 20714-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 20714-1-AP (PRKD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKD1 antibody included

经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRKD1 Ready-To-Use IHC Kit. PRKD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20714-1-AP targets PRKD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase D1
Synonyms Serine/threonine protein kinase D1, PRKCM, PKD1, PKD
Calculated Molecular Weight 102 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberNM_002742
Gene Symbol PRKD1
Gene ID (NCBI) 5587
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15139
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKD1 antibody 20714-1-APDownload protocol
IHC protocol for PRKD1 antibody 20714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols