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验证数据展示

  • WB analysis of LNCaP using 20714-1-AP

    WB analysis of LNCaP using 20714-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 20714-1-AP (PRKD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat cerebellum using 20714-1-AP

    IHC staining of rat cerebellum using 20714-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 20714-1-AP (PRKD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKD1 antibody included
  • IHC staining of rat cerebellum using 20714-1-AP

    IHC staining of rat cerebellum using 20714-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 20714-1-AP (PRKD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKD1 antibody included

PRKD1 Polyclonal antibody

PRKD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20714-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Serine/threonine protein kinase D1, PRKCM, PKD1, PKD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRKD1 Ready-To-Use IHC Kit. PRKD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20714-1-AP targets PRKD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase D1
别名 Serine/threonine protein kinase D1, PRKCM, PKD1, PKD
计算分子量 102 kDa
观测分子量100-110 kDa
GenBank蛋白编号NM_002742
基因名称 PRKD1
Gene ID (NCBI) 5587
RRIDAB_3669341
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15139
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKD1 antibody 20714-1-APDownload protocol
IHC protocol for PRKD1 antibody 20714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols