DNA-PKcs Polyclonal antibody
DNA-PKcs Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IHC detected in | mouse testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PRKDC Ready-To-Use IHC Kit. PRKDC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
28534-1-AP targets DNA-PKcs in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Immunogen | DNA-PKcs fusion protein Ag29100 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein kinase, DNA-activated, catalytic polypeptide |
| Synonyms | DNA PK catalytic subunit, DNA PKcs, DNAPK, DNPK1, HYRC, HYRC1, p350, p460, PRKDC, XRCC7 |
| Calculated Molecular Weight | 469 kDa |
| Observed Molecular Weight | 350-460 kDa |
| GenBank Accession Number | NM_006904 |
| Gene Symbol | PRKDC |
| Gene ID (NCBI) | 5591 |
| RRID | AB_2881165 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78527 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRKDC, also named as HYRC, HYRC1, DNPK1 and p460, belongs to the PI3/PI4-kinase family. PRKDC is a serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ), PRKDC is required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. It promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). It is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. It is found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. It also involved in modulation of transcription. It recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. It phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. The antibody recognizes the C-term of PRKDC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DNA-PKcs antibody 28534-1-AP | Download protocol |
| IHC protocol for DNA-PKcs antibody 28534-1-AP | Download protocol |
| IF protocol for DNA-PKcs antibody 28534-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
