验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, C2C12 cells, PC-12 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10771-1-AP targets PACT in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PACT fusion protein Ag1214 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, interferon-inducible double stranded RNA dependent activator |
| 别名 | DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX |
| 计算分子量 | 34 kDa |
| 观测分子量 | 31-34 kDa |
| GenBank蛋白编号 | BC009470 |
| 基因名称 | PRKRA |
| Gene ID (NCBI) | 8575 |
| RRID | AB_2170496 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75569 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PACT antibody 10771-1-AP | Download protocol |
| IHC protocol for PACT antibody 10771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
EMBO Rep The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis during development. | ||
Nucleic Acids Res Hepato-specific microRNA-122 facilitates accumulation of newly synthesized miRNA through regulating PRKRA. | ||
PLoS Pathog KSHV inhibits stress granule formation by viral ORF57 blocking PKR activation. | ||
Dis Model Mech A frameshift mutation in the murine Prkra gene exhibits cerebellar abnormality and reduced eIF2α phosphorylation |
