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PRKRA Monoclonal antibody, PBS Only

PRKRA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4C3

Cat no : 68518-1-PBS

Print datasheet

Synonyms

PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14

验证数据展示

  • WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

    WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
Planning an IHC experiment? We recommend our IHCeasy PRKRA Ready-To-Use IHC Kit. PRKRA primary antibody included.

产品信息

68518-1-PBS targets PRKRA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRKRA fusion protein Ag29818 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein kinase, interferon-inducible double stranded RNA dependent activator
Synonyms PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC009470
Gene Symbol PRKRA
Gene ID (NCBI) 8575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75569
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).