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验证数据展示

  • WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

    WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included
  • IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

    IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKRA antibody included

PRKRA Monoclonal antibody, PBS Only

PRKRA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68518-1-PBS

产品说明书

CloneNo. 1D4C3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PRKRA Ready-To-Use IHC Kit. PRKRA primary antibody included.

产品信息

68518-1-PBS targets PRKRA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRKRA fusion protein Ag29818 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, interferon-inducible double stranded RNA dependent activator
别名 PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC009470
基因名称 PRKRA
Gene ID (NCBI) 8575
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75569
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).