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PRKX/PRKY Polyclonal antibody

PRKX/PRKY Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17985-1-AP

Print datasheet

Synonyms

PRKY, protein kinase, Y linked

验证数据展示

  • WB analysis using 17985-1-AP

    WB analysis using 17985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17985-1-AP (PRKX/PRKY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17985-1-AP targets PRKX/PRKY in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PRKX/PRKY fusion protein Ag12404 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, Y-linked
Synonyms PRKY, protein kinase, Y linked
Calculated Molecular Weight 277 aa, 32 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC074852
Gene Symbol PRKY
Gene ID (NCBI) 5616
RRIDAB_3669286
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKY is a member of the cAMP-dependent serine-threonine protein kinase gene family, which appears to encode a protein related to the catalytic subunit of the cAMP-dependent protein kinases, which are key players in the cellular responses to the second messenger cAMP. A chromosomal aberration involving PRKY is a cause of sex reversal disorder. As a homolog with PRKX, the antibody can recognize endogenous levels of PRKY and PRKX.

实验方案

Product Specific Protocols
WB protocol for PRKX/PRKY antibody 17985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols