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验证数据展示

  • WB analysis using 67292-1-Ig

    WB analysis using 67292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67292-1-Ig

    IHC staining of human breast cancer using 67292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67292-1-Ig

    IHC staining of human breast cancer using 67292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRLR Monoclonal antibody

PRLR Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67292-1-Ig

产品说明书

CloneNo. 2E10G5
引用文献(2)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse and More (1)

应用

WB, IHC, ELISA

别名

hPRLrI, PRL R, PRLR, prolactin receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, A431 cells, T-47D cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67292-1-Ig targets PRLR in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human, mouse
文献引用反应性human
免疫原 PRLR fusion protein Ag28778 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolactin receptor
别名 hPRLrI, PRL R, PRLR, prolactin receptor
计算分子量 622 aa, 70 kDa
观测分子量 45 kDa, 70 kDa, 85-85 kDa
GenBank蛋白编号BC059392
基因名称 PRLR
Gene ID (NCBI) 5618
RRIDAB_2882557
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP16471
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).

实验方案

Product Specific Protocols
WB protocol for PRLR antibody 67292-1-IgDownload protocol
IHC protocol for PRLR antibody 67292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Genet

What Mediates Fibrosis in the Tumor Microenvironment of Clear Renal Cell Carcinoma.

Authors - Wenbo Yang
View Article
WB

Front Vet Sci

Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats.

Authors - Mengjie Zhao
View Article