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PRLR Monoclonal antibody

PRLR Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E10G5

Cat no : 67292-1-Ig

Print datasheet

Synonyms

hPRLrI, PRL R, PRLR, prolactin receptor

验证数据展示

  • WB analysis using 67292-1-Ig

    WB analysis using 67292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67292-1-Ig

    IHC staining of human breast cancer using 67292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67292-1-Ig

    IHC staining of human breast cancer using 67292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, A431 cells, T-47D cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67292-1-Ig targets PRLR in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen PRLR fusion protein Ag28778 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prolactin receptor
Synonyms hPRLrI, PRL R, PRLR, prolactin receptor
Calculated Molecular Weight 622 aa, 70 kDa
Observed Molecular Weight 45 kDa, 70 kDa, 85-85 kDa
GenBank Accession NumberBC059392
Gene Symbol PRLR
Gene ID (NCBI) 5618
RRIDAB_2882557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16471
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).

实验方案

Product Specific Protocols
WB protocol for PRLR antibody 67292-1-IgDownload protocol
IHC protocol for PRLR antibody 67292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Genet

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Authors - Wenbo Yang
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WB

Front Vet Sci

Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats.

Authors - Mengjie Zhao
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