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  • Featured Product
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PRMT1 Polyclonal antibody

PRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11279-1-AP

Print datasheet

Synonyms

Interferon receptor 1-bound protein 4, HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319

验证数据展示

  • WB analysis of A549 using 11279-1-AP

    WB analysis of A549 using 11279-1-AP

    WB result of PRMT1 antibody (11279-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT1 transfected A549 cells.

  • WB analysis using 11279-1-AP

    WB analysis using 11279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11279-1-AP

    WB analysis using 11279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11279-1-AP

    WB analysis using 11279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 11279-1-AP

    IP experiment of A549 using 11279-1-AP

    IP result of anti-PRMT1 (IP:11279-1-AP, 4ug; Detection:11279-1-AP 1:2000) with A549 cells lysate 920 ug.

  • IHC staining of human colon using 11279-1-AP

    IHC staining of human colon using 11279-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRMT1 antibody included
  • IHC staining of human colon using 11279-1-AP

    IHC staining of human colon using 11279-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRMT1 antibody included
  • IF Staining of A431 using 11279-1-AP

    IF Staining of A431 using 11279-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (11279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 11279-1-AP

    FC experiment of HepG2 using 11279-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRMT1 Polyclonal antibody (11279-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11279-1-AP targets PRMT1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PRMT1 fusion protein Ag1790 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein arginine methyltransferase 1
Synonyms Interferon receptor 1-bound protein 4, HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC019268
Gene Symbol PRMT1
Gene ID (NCBI) 3276
RRIDAB_2171319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99873
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

实验方案

Product Specific Protocols
WB protocol for PRMT1 antibody 11279-1-APDownload protocol
IHC protocol for PRMT1 antibody 11279-1-APDownload protocol
IF protocol for PRMT1 antibody 11279-1-APDownload protocol
IP protocol for PRMT1 antibody 11279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IP

Cell Metab

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners

Authors - Qing Li
View Article
mouseWB,IP

Nucleic Acids Res

RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility

Authors - Heejin Choi
View Article
humanWB

Cell Death Dis

PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis

Authors - Hao Liu
View Article
humanIHC

Oncogene

PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.

Authors - Xin-Ke Yin
View Article
human,mouseWB,IHC

NPJ Precis Oncol

The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer

Authors - Xiangwei Zeng
View Article
mouseWB

Diabetes

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Authors - Yi Zhong
View Article