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验证数据展示

  • WB analysis using 68534-1-Ig

    WB analysis using 68534-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68534-1-Ig (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PRMT5 Monoclonal antibody

PRMT5 Monoclonal Antibody for WB, ELISA
Cat No. 68534-1-Ig

Print datasheet

CloneNo. 4A1B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, ELISA

Synonyms

72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy PRMT5 Ready-To-Use IHC Kit. PRMT5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68534-1-Ig targets PRMT5 in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, rat
Immunogen PRMT5 fusion protein Ag9678 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein arginine methyltransferase 5
Synonyms 72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs
Calculated Molecular Weight 637 aa, 73 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC025979
Gene Symbol PRMT5
Gene ID (NCBI) 10419
RRIDAB_3085242
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14744
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for PRMT5 antibody 68534-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols