验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells |
Planning an IHC experiment? We recommend our IHCeasy PRMT5 Ready-To-Use IHC Kit. PRMT5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68534-1-Ig targets PRMT5 in WB, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, rat |
| 免疫原 | PRMT5 fusion protein Ag9678 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein arginine methyltransferase 5 |
| 别名 | 72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs |
| 计算分子量 | 637 aa, 73 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC025979 |
| 基因名称 | PRMT5 |
| Gene ID (NCBI) | 10419 |
| RRID | AB_3085242 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O14744 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRMT5 antibody 68534-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |