验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, HepG2 cells, Jurkat cells |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67669-1-Ig targets PRMT7 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, rat |
| 文献引用反应性 | human |
| 免疫原 | PRMT7 fusion protein Ag30434 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein arginine methyltransferase 7 |
| 别名 | FLJ10640, KIAA1933, PRMT7 |
| 计算分子量 | 78 kDa |
| 观测分子量 | 78 kDa |
| GenBank蛋白编号 | BC000146 |
| 基因名称 | PRMT7 |
| Gene ID (NCBI) | 54496 |
| RRID | AB_2882865 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9NVM4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRMT7 antibody 67669-1-Ig | Download protocol |
| IF protocol for PRMT7 antibody 67669-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression | ||
Gastroenterology Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms |
