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验证数据展示

  • WB analysis of mouse liver using 25382-1-AP

    WB analysis of mouse liver using 25382-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25382-1-AP

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 25382-1-AP

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 25382-1-AP

    IF Staining of HeLa using 25382-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PROC antibody (25382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PROC Polyclonal antibody

PROC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25382-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25382-1-AP targets PROC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 PROC fusion protein Ag11328 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein C (inactivator of coagulation factors Va and VIIIa)
别名 Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA
计算分子量 461 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC034377
基因名称 PROC
Gene ID (NCBI) 5624
RRIDAB_2880052
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04070
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROC belongs to the peptidase S1 family. PROC is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PMID:25618265). It exerts a protective effect on the endothelial cell barrier function (PMID:25651845).

实验方案

Product Specific Protocols
WB protocol for PROC antibody 25382-1-APDownload protocol
IHC protocol for PROC antibody 25382-1-APDownload protocol
IF protocol for PROC antibody 25382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article