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PROC Polyclonal antibody

PROC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25382-1-AP

Print datasheet

Synonyms

Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA

验证数据展示

  • WB analysis of mouse liver using 25382-1-AP

    WB analysis of mouse liver using 25382-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25382-1-AP

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 25382-1-AP

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 25382-1-AP

    IF Staining of HeLa using 25382-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PROC antibody (25382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

25382-1-AP targets PROC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PROC fusion protein Ag11328 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein C (inactivator of coagulation factors Va and VIIIa)
Synonyms Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA
Calculated Molecular Weight 461 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC034377
Gene Symbol PROC
Gene ID (NCBI) 5624
RRIDAB_2880052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04070
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROC belongs to the peptidase S1 family. PROC is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PMID:25618265). It exerts a protective effect on the endothelial cell barrier function (PMID:25651845).

实验方案

Product Specific Protocols
WB protocol for PROC antibody 25382-1-APDownload protocol
IHC protocol for PROC antibody 25382-1-APDownload protocol
IF protocol for PROC antibody 25382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article