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验证数据展示

  • WB analysis of human spleen using 11391-1-AP

    WB analysis of human spleen using 11391-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11391-1-AP (EPCR/CD201 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11391-1-AP

    IHC staining of human pancreas cancer using 11391-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11391-1-AP (EPCR/CD201 antibody at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas cancer using 11391-1-AP

    IHC staining of human pancreas cancer using 11391-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11391-1-AP (EPCR/CD201 antibody at dilution of 1:100 (under 40x lens).

EPCR/CD201 Polyclonal antibody

EPCR/CD201 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11391-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD201, EPCR, PROCR, CCD41, CCCA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman spleen tissue
Positive IHC detected inhuman pancreas cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11391-1-AP targets EPCR/CD201 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 EPCR/CD201 fusion protein Ag1937 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein C receptor, endothelial (EPCR)
别名 CD201, EPCR, PROCR, CCD41, CCCA
计算分子量 238 aa, 27 kDa
观测分子量 49 kDa
GenBank蛋白编号BC014451
基因名称 PROCR
Gene ID (NCBI) 10544
RRIDAB_2268938
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective effects on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424).

实验方案

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 11391-1-APDownload protocol
IHC protocol for EPCR/CD201 antibody 11391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Analyst

An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.

Authors - Yu Yanyan Y
View Article