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PRODH Polyclonal antibody

PRODH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22980-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

HSPOX2, p53 induced gene 6 protein, PIG6, POX, POX2, PRODH, PRODH1, Proline oxidase, Proline oxidase 2, SCZD4, TP53I6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, A549 cells, rat liver tissue
Positive IHC detected inhuman cerebellum tissue, human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22980-1-AP targets PRODH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 PRODH fusion protein Ag18429 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline dehydrogenase (oxidase) 1
别名 HSPOX2, p53 induced gene 6 protein, PIG6, POX, POX2, PRODH, PRODH1, Proline oxidase, Proline oxidase 2, SCZD4, TP53I6
计算分子量 600 aa, 68 kDa
观测分子量 56 kDa, 66 kDa
GenBank蛋白编号BC118597
基因名称 PRODH
Gene ID (NCBI) 5625
RRIDAB_2879191
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43272
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRODH(Proline dehydrogenase 1, mitochondrial) is also named as PIG6, HSPOX2, PRODH1, PRODH2, POX, SCZD4, TP53I6 and belongs to the proline oxidase family. It is an oxidoreductase involved in the transfer of redox potential across the mitochondrial membrane and catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C). High PRODH activity is sufficient to induce mitochondria-mediated apoptosis in the presence of proline. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) and defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). This protein has 3 isoforms produced by alternative splicing with the molecular mass of 68 kDa, 59 kDa and 56 kDa.

实验方案

Product Specific Protocols
WB protocol for PRODH antibody 22980-1-APDownload protocol
IHC protocol for PRODH antibody 22980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress.

Authors - Jagannath Misra
humanWB

Cell Rep

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells

Authors - Debanik Choudhury
ratWB

Oxid Med Cell Longev

Metabolomic Analysis of the Ameliorative Effect of Enhanced Proline Metabolism on Hypoxia-Induced Injury in Cardiomyocytes.

Authors - Jiacheng Wang
  • KD Validated
humanWB

Amino Acids

PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Authors - Chunhong Cui
humanWB

Amino Acids

Activation of proline metabolism maintains ATP levels during cocaine-induced polyADP-ribosylation.

Authors - Sabyasachi Dash
  • KD Validated
WB,IF

Animals (Basel)

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine.

Authors - Chengwen Feng
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