验证数据展示
经过测试的应用
Positive WB detected in | mouse kidney tissue, human testis tissue, mouse testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31047-1-AP targets PRODH2 in WB, ELISA applications and shows reactivity with Human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse |
免疫原 | PRODH2 fusion protein Ag33970 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | proline dehydrogenase (oxidase) 2 |
别名 | HSPOX1, Probable proline oxidase 2, PRODH2 |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC166629 |
基因名称 | PRODH2 |
Gene ID (NCBI) | 58510 |
RRID | AB_3669831 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9UF12 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRODH2 is a dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. It can also use proline as a substrate but with a very much lower efficiency (PMID: 25697095).
实验方案
Product Specific Protocols | |
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WB protocol for PRODH2 antibody 31047-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |