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验证数据展示

  • IHC staining of human colon cancer using 15152-1-AP

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15152-1-AP

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 15152-1-AP

    IHC staining of mouse kidney using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 15152-1-AP

    IHC staining of mouse kidney using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15152-1-AP

    IHC staining of mouse liver using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15152-1-AP

    IHC staining of mouse liver using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15152-1-AP

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15152-1-AP

    IHC staining of human colon cancer using 15152-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15152-1-AP (Prokineticin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of NIH/3T3 using 15152-1-AP

    FC experiment of NIH/3T3 using 15152-1-AP

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human Prokineticin 1 (15152-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using 15152-1-AP

    IF Staining of NIH/3T3 using 15152-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Prokineticin 1 antibody (15152-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Prokineticin 1 Polyclonal antibody

Prokineticin 1 Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 15152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

PROK1, Mambakine, Endocrine-gland-derived vascular endothelial growth factor, EG-VEGF, EGVEGF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15152-1-AP targets Prokineticin 1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Prokineticin 1 fusion protein Ag5098 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prokineticin 1
别名 PROK1, Mambakine, Endocrine-gland-derived vascular endothelial growth factor, EG-VEGF, EGVEGF
计算分子量 12 kDa
GenBank蛋白编号BC025399
基因名称 Prokineticin 1
Gene ID (NCBI) 84432
RRIDAB_3669199
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP58294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Prokineticin 1 (PROK1) is also named as EG-VEGF and Mambakine, belongs to the to the AVIT (prokineticin) family. Prokineticin signaling comprises two secreted proteins (Prok-1 and Prok-2) and two cognate G-protein coupled receptors (PK-R1 and PK-R2) that are widely expressed in different tissues and of great versatility. Prokineticins were shown to promote angiogenesis in steroidgenic glands, heart and reproductive organs (PMID:18440852). PROK1 has been described as a secretory protein with pleiotropic functions and as a novel tissue-specific angiogenic factor (PMID:32355954). EG-VEGF/PK-1, described as selective angiogenic mitogen, is widely expressed in different tissues including steroidogenic endocrine glands (PMID:16320832). A lot of data suggests EG-VEGF to be restricted to endocrine glands and to some endocrine-dependent organs (PMID:28386275).

实验方案

Product Specific Protocols
IHC protocol for Prokineticin 1 antibody 15152-1-APDownload protocol
IF protocol for Prokineticin 1 antibody 15152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols