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CD133-1,2 Polyclonal antibody

CD133-1,2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19946-1-AP

Print datasheet

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

验证数据展示

  • WB analysis of HT-29 using 19946-1-AP

    WB analysis of HT-29 using 19946-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 19946-1-AP (CD133-1,2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 19946-1-AP

    IP experiment of Y79 using 19946-1-AP

    IP result of anti-CD133-1,2 (IP:19946-1-AP, 4ug; Detection:19946-1-AP 1:300) with Y79 cells lysate 2000ug.

经过测试的应用

Positive WB detected inHT-29 cells, Caco-2 cells
Positive IP detected inY79 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19946-1-AP targets CD133-1,2 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prominin 1
Synonyms AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberNM_006017
Gene Symbol CD133
Gene ID (NCBI) 8842
RRIDAB_10644275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2 of CD133.

实验方案

Product Specific Protocols
WB protocol for CD133-1,2 antibody 19946-1-APDownload protocol
IP protocol for CD133-1,2 antibody 19946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols