CD133 Polyclonal antibody

CD133 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18495-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

PROM1, MSTP061, MCDR2, CORD12, Antigen AC133

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCaco-2 cells, mouse skeletal muscle tissue, HT-29 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18495-1-AP targets CD133 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 CD133 fusion protein Ag13327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prominin 1
别名 PROM1, MSTP061, MCDR2, CORD12, Antigen AC133
计算分子量 97 kDa
观测分子量 110-115 kDa
GenBank蛋白编号BC012089
基因名称 CD133
Gene ID (NCBI) 8842
RRIDAB_2172858
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors.

实验方案

Product Specific Protocols
WB protocol for CD133 antibody 18495-1-APDownload protocol
IHC protocol for CD133 antibody 18495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

USP7 regulates growth and maintains the stemness of p53-mutant colorectal cancer cells via stabilizing of mutant p53

Authors - Xue Li
human,mouseWB,IF

J Oncol

The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses

Authors - Chang-Jun Liu
humanWB

Cancer Med

Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma

Authors - Zhen Fang
humanIF

Oncol Lett

Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation

Authors - Yufei Li
humanWB

World J Oncol

DNA Damage-Induced Apoptosis Suppressor Triggers Progression and Stemness of Glioma by Enhancing Lymphoid Enhancer-Binding Factor 1 Expression

Authors - You Lin Chen
human,mouseWB

Bioorg Med Chem

Synthesis and anti-tumor activity of menthylamine derivatives: Focusing on the potent inhibitory effect of compound W8 on glioma growth

Authors - Xiaoran Wu
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