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PROS1 Recombinant antibody

PROS1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241241E5

Cat no : 84107-2-RR

Print datasheet

Synonyms

Vitamin K-dependent protein S, PROS, 241241E5

验证数据展示

  • WB analysis using 84107-2-RR

    WB analysis using 84107-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84107-2-RR (PROS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 84107-2-RR

    FC experiment of A549 using 84107-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PROS1 Recombinant antibody (84107-2-RR, Clone:241241E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84107-2-RR

    Affinity and Kinetic Characterization of 84107-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84107-2-RR against Human PROS1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inDU 145 cells, PC-3 cells, HuH-7 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84107-2-RR is PROS1 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen PROS1 fusion protein Ag10539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein S (alpha)
Synonyms Vitamin K-dependent protein S, PROS, 241241E5
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC015801
Gene Symbol PROS1
Gene ID (NCBI) 5627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07225
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROS1, also named as Vitamin K-dependent protein S, is a 676 amino acid protein, which contains 4 EGF-like domains, contains one Gla (gamma-carboxy-glutamate) domain and contains 2 laminin G-like domains. PROS1 as a secreted protein platelets alpha granules is expressed in plasma. PROS1 is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa and helps to prevent coagulation and stimulating fibrinolysis.

实验方案

Product Specific Protocols
WB protocol for PROS1 antibody 84107-2-RRDownload protocol
FC protocol for PROS1 antibody 84107-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols