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PROX1 Recombinant antibody

PROX1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H12

Cat no : 81778-1-RR

Print datasheet

Synonyms

PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 2H12

验证数据展示

  • WB analysis using 81778-1-RR

    WB analysis using 81778-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81778-1-RR (PROX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HuH-7 using 81778-1-RR

    IF Staining of HuH-7 using 81778-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (81778-1-RR, Clone: 2H12 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), PROX1 antibody (67438-1-Ig, Clone: 1D4B1, red).

  • Affinity and Kinetic Characterization of 81778-1-RR

    Affinity and Kinetic Characterization of 81778-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81778-1-RR against Human PROX1 were performed. The affinity constant is 26.4 nM.

经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, SH-SY5Y cells
Positive IF/ICC detected inHuH-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81778-1-RR targets PROX1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PROX1 fusion protein Ag1543 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name prospero homeobox 1
Synonyms PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 2H12
Calculated Molecular Weight 737 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC024201
Gene Symbol PROX1
Gene ID (NCBI) 5629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

实验方案

Product Specific Protocols
WB protocol for PROX1 antibody 81778-1-RRDownload protocol
IF protocol for PROX1 antibody 81778-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols