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验证数据展示

  • WB analysis using 81778-1-RR

    WB analysis using 81778-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81778-1-RR (PROX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HuH-7 using 81778-1-RR

    IF Staining of HuH-7 using 81778-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (81778-1-RR, Clone: 2H12 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), PROX1 antibody (67438-1-Ig, Clone: 1D4B1, red).

PROX1 Recombinant antibody

PROX1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81778-1-RR

Print datasheet

CloneNo. 2H12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 2H12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, SH-SY5Y cells
Positive IF/ICC detected inHuH-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81778-1-RR targets PROX1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen PROX1 fusion protein Ag1543 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name prospero homeobox 1
Synonyms PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 2H12
Calculated Molecular Weight 737 aa, 83 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC024201
Gene Symbol PROX1
Gene ID (NCBI) 5629
RRIDAB_2935579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92786
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

实验方案

Product Specific Protocols
WB protocol for PROX1 antibody 81778-1-RRDownload protocol
IF protocol for PROX1 antibody 81778-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols