验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
10106-1-AP targets PRPF3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PRPF3 fusion protein Ag0154 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) |
| 别名 | RP18, Prp3p, PRP3, Pre-mRNA-splicing factor 3, HPRP3P |
| 计算分子量 | 78 kDa |
| 观测分子量 | 90 kDa, 78 kDa |
| GenBank蛋白编号 | BC000184 |
| 基因名称 | PRPF3 |
| Gene ID (NCBI) | 9129 |
| RRID | AB_2170994 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43395 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPF3 antibody 10106-1-AP | Download protocol |
| IHC protocol for PRPF3 antibody 10106-1-AP | Download protocol |
| IP protocol for PRPF3 antibody 10106-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Mol Genet PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa. | ||
Mol Cell Biol A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis. | ||
J Biol Chem PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA. | ||
Cell Mol Biol Lett TMEM43 promotes pancreatic cancer progression by stabilizing PRPF3 and regulating RAP2B/ERK axis. | ||
Food Funct Flaxseed oil attenuates PFOS-induced testicular damage by regulating RNA alternative splicing |
