验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23901-1-AP targets PRPF38B in WB, ELISA applications and shows reactivity with human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, Mouse |
| 免疫原 |
CatNo: Ag20983 Product name: Recombinant human PRPF38B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC130346 Sequence: MANNSPALTGNSQPQHQAAAAAAQQQQQCGGGGATKPAVSGKQGNVLPLWGNEKTMNLNPMILTNILSSPYFKVQLYELKTYHEVVDEIYFKVTHVEPWEKGSRKTAGQTGMCGGVRGVGTGGIVSTAFCLLYKLFTLKLTRKQVMGLITHTDSPYIRALGFMYIRYTQPPTDLWDWFESFLDDEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PRP38 pre-mRNA processing factor 38 (yeast) domain containing B |
| 别名 | Pre mRNA splicing factor 38B, PRPF38B, RP11 293A10.1, Sarcoma antigen NY SAR 27 |
| 计算分子量 | 546 aa, 64 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC130346 |
| 基因名称 | PRPF38B |
| Gene ID (NCBI) | 55119 |
| RRID | AB_3085715 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q5VTL8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRPF38B (Pre-mRNA Processing Factor 38B) is a unique component of the U4/U6.U5 tri small nuclear ribonuclear protein (snRNP) particle and is necessary for an essential step in late spliceosome maturation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPF38B antibody 23901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
