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PRPF39 Polyclonal antibody

PRPF39 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24480-1-AP

Print datasheet

Synonyms

Pre mRNA processing factor 39, PRP39 homolog, PRPF39

验证数据展示

  • WB analysis of K-562 using 24480-1-AP

    WB analysis of K-562 using 24480-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24480-1-AP

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells, COLO 320 cells, Raji cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24480-1-AP is PRPF39 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen PRPF39 fusion protein Ag21683 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae)
Synonyms Pre mRNA processing factor 39, PRP39 homolog, PRPF39
Calculated Molecular Weight 669 aa, 78 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC125126
Gene Symbol PRPF39
Gene ID (NCBI) 55015
RRIDAB_2879563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UA1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRPF39 also termed as Pre mRNA processing factor 39 or PRP39 homolog is a 669 amino acid protein, which contains 7 HAT repeats and belongs to the PRP39 family. PRPF39 localizes in nucleus and may play a role in mRNA splicing.

实验方案

Product Specific Protocols
WB protocol for PRPF39 antibody 24480-1-APDownload protocol
IHC protocol for PRPF39 antibody 24480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols