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验证数据展示

  • WB analysis of HEK-293 using 17392-1-AP

    WB analysis of HEK-293 using 17392-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 17392-1-AP

    IP experiment of HEK-293 using 17392-1-AP

    IP result of anti-PRPF40A (IP:17392-1-AP, 4ug; Detection:17392-1-AP 1:500) with HEK-293 cells lysate 3000ug.

PRPF40A Polyclonal antibody

PRPF40A Polyclonal Antibody for WB, IP, ELISA
Cat No. 17392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Fas ligand associated factor 1, FBP 11, FBP11, FLAF1, FNBP3, Formin binding protein 11, Formin binding protein 3, HIP 10, HIP10, Huntingtin yeast partner A, HYPA, NY REN 6, PRPF40A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PRPF40A Ready-To-Use IHC Kit. PRPF40A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17392-1-AP targets PRPF40A in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 PRPF40A fusion protein Ag11290 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)
别名 Fas ligand associated factor 1, FBP 11, FBP11, FLAF1, FNBP3, Formin binding protein 11, Formin binding protein 3, HIP 10, HIP10, Huntingtin yeast partner A, HYPA, NY REN 6, PRPF40A
计算分子量 957 aa, 109 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC027178
基因名称 PRPF40A
Gene ID (NCBI) 55660
RRIDAB_2171031
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75400
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRPF40A antibody 17392-1-APDownload protocol
IP protocol for PRPF40A antibody 17392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols