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  • Featured Product
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PRPF40B Polyclonal antibody

PRPF40B Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16929-1-AP

Print datasheet

Synonyms

Huntingtin yeast partner C, HYPC, PRPF40B

验证数据展示

  • WB analysis of Y79 using 16929-1-AP

    WB analysis of Y79 using 16929-1-AP

    WB result of PRPF40B antibody (16929-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRPF40B transfected Y79 cells.

  • IP experiment of Y79 using 16929-1-AP

    IP experiment of Y79 using 16929-1-AP

    IP result of anti-PRPF40B (IP:16929-1-AP, 4ug; Detection:16929-1-AP 1:5000) with Y79 cells lysate 2800 ug.

  • WB analysis using 16929-1-AP

    WB analysis using 16929-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16929-1-AP (PRPF40B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inY79 cells, K-562 cells, mouse testis tissue
Positive IP detected inY79 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16929-1-AP targets PRPF40B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PRPF40B fusion protein Ag10361 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PRP40 pre-mRNA processing factor 40 homolog B (S. cerevisiae)
Synonyms Huntingtin yeast partner C, HYPC, PRPF40B
Calculated Molecular Weight 871 aa, 99 kDa
Observed Molecular Weight130-150 kDa
GenBank Accession NumberBC067364
Gene Symbol PRPF40B
Gene ID (NCBI) 25766
RRIDAB_2171041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NWY9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRPF40B antibody 16929-1-APDownload protocol
IP protocol for PRPF40B antibody 16929-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols