PRPF8 Polyclonal antibody
PRPF8 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, HeLa cells, human placenta tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11171-1-AP targets PRPF8 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IP, RIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | PRPF8 fusion protein Ag1652 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae) |
| Synonyms | PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, p220, HPRP8 |
| Calculated Molecular Weight | 274 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC064370 |
| Gene Symbol | PRPF8 |
| Gene ID (NCBI) | 10594 |
| RRID | AB_2171179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P2Q9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRPF8 antibody 11171-1-AP | Download protocol |
| IHC protocol for PRPF8 antibody 11171-1-AP | Download protocol |
| IP protocol for PRPF8 antibody 11171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. | ||
Nucleic Acids Res Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway. | ||
Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. | ||
J Biol Chem Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly. |
