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验证数据展示

  • WB analysis using 17399-1-AP

    WB analysis using 17399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17399-1-AP

    IHC staining of mouse brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of PC-12 using 17399-1-AP

    FC experiment of PC-12 using 17399-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of mouse colon using 17399-1-AP

    WB analysis of mouse colon using 17399-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 17399-1-AP

    IHC staining of rat brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human colon using 17399-1-AP

    IF Staining of human colon using 17399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human colon using 17399-1-AP

    IF Staining of human colon using 17399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Peripherin Polyclonal antibody

Peripherin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA
Cat No. 17399-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

别名

peripherin, PRPH, NEF4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, COLO 320 cells, human colon tissue, rat colon tissue, rat spinal cord tissue
Positive IHC detected inmouse brain tissue, human colon tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon tissue
Positive FC (Intra) detected inPC-12 cells
Positive FC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

17399-1-AP targets Peripherin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 Peripherin fusion protein Ag11365 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peripherin
别名 peripherin, PRPH, NEF4
计算分子量 470 aa, 54 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC032703
基因名称 Peripherin
Gene ID (NCBI) 5630
RRIDAB_2171195
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41219
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

实验方案

Product Specific Protocols
WB protocol for Peripherin antibody 17399-1-APDownload protocol
IHC protocol for Peripherin antibody 17399-1-APDownload protocol
IF protocol for Peripherin antibody 17399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

Biotechniques

Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.

Authors - Xia Xiao
View Article