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PRPSAP1 Polyclonal antibody

PRPSAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16790-1-AP

Print datasheet

Synonyms

PAP39, PRPSAP1

验证数据展示

  • IHC staining of human liver cancer using 16790-1-AP

    IHC staining of human liver cancer using 16790-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16790-1-AP (PRPSAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRPSAP1 antibody included
  • WB analysis of human testis using 16790-1-AP

    WB analysis of human testis using 16790-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16790-1-AP (PRPSAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 16790-1-AP

    IP experiment of mouse testis using 16790-1-AP

    IP result of anti-PRPSAP1 (IP:16790-1-AP, 4ug; Detection:16790-1-AP 1:300) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRPSAP1 Ready-To-Use IHC Kit. PRPSAP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16790-1-AP targets PRPSAP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PRPSAP1 fusion protein Ag10227 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoribosyl pyrophosphate synthetase-associated protein 1
Synonyms PAP39, PRPSAP1
Calculated Molecular Weight 356 aa, 39 kDa
GenBank Accession NumberBC009012
Gene Symbol PRPSAP1
Gene ID (NCBI) 5635
RRIDAB_2171534
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRPSAP1 antibody 16790-1-APDownload protocol
IHC protocol for PRPSAP1 antibody 16790-1-APDownload protocol
IP protocol for PRPSAP1 antibody 16790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols