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PRR14 Polyclonal antibody
PRR14 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HL-60 cells, MCF-7 cells, SW480 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22819-1-AP targets PRR14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | PRR14 fusion protein Ag14119 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | proline rich 14 |
| Synonyms | Proline-rich protein 14, Proline rich protein 14, proline rich 14 |
| Calculated Molecular Weight | 585 aa, 64 kDa |
| Observed Molecular Weight | 64-70 kDa |
| GenBank Accession Number | BC000119 |
| Gene Symbol | PRR14 |
| Gene ID (NCBI) | 78994 |
| RRID | AB_2879174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRR14 (proline-rich protein 14) is a 585-amino acid protein with a calculated molecular weight of 64 kDa. This protein functions to tether heterochromatin to the nuclear periphery during interphase, through associations with heterochromatin protein 1 (HP1) and the nuclear lamina. After disassembly early in mitosis, PRR14 reassembles in two steps, first binding to anaphase chromosomes through HP1, followed by association with the nuclear lamina in telophase. PRR14 may thereby play a role in specifying HP1-bound heterochromatin for reattachment to the nuclear lamina at mitotic exit. (PMID: 24209742; 24637393)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRR14 antibody 22819-1-AP | Download protocol |
| IHC protocol for PRR14 antibody 22819-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
